Course objectives - The analytical procedure - Classification of analytical methods - Materials - Analytical balance - Introduction to statistics: error, significant numbers and rounding, accuracy and precision, standard deviation and confidence limits - Statistical evaluation of analytical data - Unit of concentration.
Introduction to acids and bases, acid-base equilibria, neutralization titrations, pH calculation during titration, acid-base titrations in non-aqueous environment - Introduction to complexes, complexometric titrations, titrations with EDTA - Introduction to oxidation-reduction reactions , potential standards, Nernst equation, factors that modify the standard potential: conditional potentials, redox titrations - Precipitation titrations, argentometry: Mohr method, Volhard and Fajans - Indicator Theory.
Introduction to the slightly soluble compounds, solubility product, solubility and factors that influence solubility. Fractional precipitation. Gravimetry: precipitation reagents, calculations in gravimetric analysis: gravimetric analytical factor (f.a.g.).
Methods for the determination of water content: Gravimetric method (direct and indirect), Azeotropic Distillation, Karl Fischer Reagent.
Chromatographic and spectroscopic methods
Principles of chromatography - liquid chromatography, HPLC - gas chromatography - separation of active ingredients and their quantitative determination.
Laboratory notebook - Calculations in volumetric analysis - Correction factor - Titrated solutions of alkaline hydroxides - Standardization of a 0,1 N solution of sodium hydroxide with oxalic acid dihydrate - Determination of the purity of a substance (citric acid, tartaric acid, acid boric acid) - Titrated solutions of acids: preparation of a normal solution of hydrochloric acid and sulfuric acid - Standardization of a 0,1 N solution of HCl with a 0,1 N solution of NaOH (different indicators) and with sodium carbonate - Dosage of aspirin - Preparation of a solution by known titre of EDTA and standardization with calcium carbonate - Dosing with EDTA of zinc, magnesium and bismuth - Preparation and standardization of a solution of KMnO4 - Dosage of hydrogen peroxide - Dosing iron (II).