The IR region of electromagnetic spectrum.Absorptions of organic molecules. Vibrational modes and bond stretchings in IR. Spectrometer design and its components. Sample preparation. Presentation of IR spectra. Interpretation of IR spectra of organic molecules according to their functional groups. FT IR.
The physical basis of NMR Spectroscopy. The chemical shift: 1H and 13C chemical shifts of organic compounds. Equivalence, Simmetry and chirality; homotopic, enantiotopic and diastereotopic groups. Indirect spin-spin coupling: H,H coupling constants and chemical structure; C,H coupling constants and chemical structure; C,C coupling constants and chemical structure; coupling mechanism. Systematic Notation for Spin Systems. Chemical and magnetic equivalence. Double resonance experiments: spin decoupling in 1H NMR spectroscopy and in 13C NMR Spectroscopy. Assignment of 1H and 13C signals. Relaxation: spin-lattice relaxation of 13C nuclei; spin-spin relaxation. One-Dimensional NMR experiments: simple pulse experiments; the J-modulated spin-echo experiment; signal enhancement by polarization transfer; the DEPT experiment. Two-Dimensional NMR Spectroscopy: the two-dimensional NMR experiment; Two-Dimensional Correlated NMR Spectroscopy; the Two-Dimensional INADEQUATE Experiment. The Nuclear Overhauser Effect. The NOESY experiment. Dynamic NMR Spectroscopy: introduction and applications. Shift Reagents: Lanthanide Shift Reagents, fundamentals, applications and quantitative interpretation. Chiral lanthanide shift reagents. Chiral solvents.
Fundamentals of Organic Mass Spectrometry. Mass spectrum. Ions types in Organic Mass Spectrometry. Mass Analyzers. Single-focusing or Double-focusing Mass Analyzers. Time-of-flight MS. Ionization: electron impact ionization (EI); Chemical Ionization (CI); Fast Atom Bombardment (FAB); MALDI. Metastable Ions. GC-MS and HPLC-MS. Determination of Molecolar Weight and Elemental Composition. Determination of molecular formula from high-resolution MS Data.